| Metabolic and Phylogenetic Profile of Bacterial Community in Guishan Coastal Water（Pearl River Estuary）, South China Sea |
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| 作者姓名： | HU Xiaojuan, LIU Qing, LI Zhuojia, HE Zhili, GONG Yingxue, CAO Yucheng, YANG Yufeng |
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| 作者单位： | [1]Institute of Hydrobiology, College of Life Science and Technology, Jinan University, Guangzhou 510632, P. R. China; [2]South China Sea Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, P. R. China; [3]Institute of Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA; [4]Research Center for Molecular Biology, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, P. R. China; [5]Key Laboratory of Eutrophication and Red Tide Prevention, Education Department of Guangdong Province, Guangzhou 510632, P. R. China |
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| 基金项目： | supported by the Support Project of Science and Technology of China （Grant No. 2012BAD18B01）; Special Fund for Agro-scientific Research in the Public Interest （201403008）; the Natural Science Foundation of China （Grant Nos. U1301235, 41173079）; special scientific research funds for central non-profit institutes,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences （2014TS04） |
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| 摘 要： | Characteristics of a microbial community are important as they indicate the status of aquatic ecosystems. In the present study, the metabolic and phylogenetic profile of the bacterioplankton community in Guishan coastal water（Pearl River Estuary）, South China Sea, at 12 sites（S1–S12） were explored by community-level physiological profiling（CLPP） with BIOLOG Eco-plate and denaturing gradient gel electrophoresis（DGGE）. Our results showed that the core mariculture area（S6, S7 and S8） and the sites associating with human activity and sewage discharge（S11 and S12） had higher microbial metabolic capability and bacterial community diversity than others（S1–5, S9–10）. Especially, the diversity index of S11 and S12 calculated from both CLPP and DGGE data（H 3.2） was higher than that of others as sewage discharge may increase water nitrogen and phosphorus nutrient. The bacterial community structure of S6, S8, S11 and S12 was greatly influenced by total phosphorous, salinity and total nitrogen. Based on DGGE fingerprinting, proteobacteria, especially γ- and α-proteobacteria, were found dominant at all sites. In conclusion, the aquaculture area and wharf had high microbial metabolic capability. The structure and composition of bacterial community were closely related to the level of phosphorus, salinity and nitrogen.
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| 关 键 词： | 细菌群落 沿海水域 新陈代谢 中国南海 珠江口 变性梯度凝胶电泳 水生生态系统 进化 |
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