| 光生物反应器中环境因子对蛋白核小球藻(Chlorella pyrenoidosa)生长和油脂积累的影响 |
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| 引用本文： | 丛 峰,孙 雪,徐年军,蔡西栗.光生物反应器中环境因子对蛋白核小球藻(Chlorella pyrenoidosa)生长和油脂积累的影响[J].海洋与湖沼,2013,44(5):1295-1230. |
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| 作者姓名： | 丛 峰 孙 雪 徐年军 蔡西栗 |
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| 作者单位： | 宁波大学 应用海洋生物技术教育部重点实验室和浙江省海洋生物工程重点实验室;宁波大学 应用海洋生物技术教育部重点实验室和浙江省海洋生物工程重点实验室;宁波大学 应用海洋生物技术教育部重点实验室和浙江省海洋生物工程重点实验室;宁波大学 应用海洋生物技术教育部重点实验室和浙江省海洋生物工程重点实验室 |
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| 基金项目： | 浙江省科技厅公益性研究项目, 2010C33066号; 浙江省创新团队项目, 2009R50012-6号; 宁波市科技攻关项目, 2010C10022号; 宁波市科技局国际合作项目, 2010D10012号。 |
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| 摘 要： | 采用气升式光生物反应器培养系统, 进行了通气速率、氮浓度、Fe3+浓度、IAA浓度、CO2补充量等环境因子对蛋白核小球藻生长和油脂积累影响的研究。结果表明, 通气速率为2.0L/min时小球藻的生长速率和油脂产量最大分别为6.17mg/(L·d)和1.77mg/(L·d); 实验范围内氮浓度对小球藻油脂产量影响规律性不强; Fe3+浓度为0.1mmol/L时小球藻的生长速率最大为7.29mg/(L·d), Fe3+浓度为1.0mmol/L时小球藻油脂产量最高为2.54mg/(L·d); IAA浓度为1.0mg/L时小球藻的生长速率为7.47mg/(L·d), 油脂产量为2.89mg/(L·d), 二者都达到最大值; CO2补充量为6000mL/d时小球藻的生长速率最大为9.88mg/(L·d), 补充量为4000mL/d时小球藻油脂产量最高为3.86mg/(L·d)。经过条件优化, 小球藻油脂产量得到了显著的提高。
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| 关 键 词： | 蛋白核小球藻 光生物反应器 环境因子 生长速率 油脂积累 |
| 收稿时间： | 2013/2/26 0:00:00 |
| 修稿时间： | 2013/5/16 0:00:00 |
EFFECTS OF ENVIRONMENTAL FACTORS ON GROWTH AND LIPID ACCUMULATION OF CHLORELLA PYRENOIDOSA IN PHOTOBIOREACTOR |
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| CONG Feng,SUN Xue,XU Nian-Jun and CAI Xi-Li.EFFECTS OF ENVIRONMENTAL FACTORS ON GROWTH AND LIPID ACCUMULATION OF CHLORELLA PYRENOIDOSA IN PHOTOBIOREACTOR[J].Oceanologia Et Limnologia Sinica,2013,44(5):1295-1230. |
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| Authors: | CONG Feng SUN Xue XU Nian-Jun and CAI Xi-Li |
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| Institution: | Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University,Marine Biotechnology Key Laboratory of Zhejiang Province;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University,Marine Biotechnology Key Laboratory of Zhejiang Province;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University,Marine Biotechnology Key Laboratory of Zhejiang Province;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University,Marine Biotechnology Key Laboratory of Zhejiang Province |
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| Abstract: | The effects of air aeration rate, nitrogen, Fe3+, IAA concentration, and CO2 supply volume on growth and lipid accumulation of microalgae Chlorella pyrenoidosa in airlift photobioreactor were studied. Results show that when the air aeration rate was 2.0L/min, the growth rate of C. pyrenoidosa was 6.17mg/(L·d) and the lipid production was 1.77mg/(L·d). During the experimental time, the nitrogen concentration did not affect the algal lipid production significantly. The optimal conditions of other factors were: Fe3+ concentration at 0.1mmol/L for the best growth rate 7.29mg/(L·d)], and 1.0mmol/L for best lipid production 2.54mg/(L·d)]; IAA concentration at 1.0mg/L for both the growth rate and lipid production 7.47 and 2.89mg/(L·d), respectively]; CO2 supply volume at 6000mL/d for the growth rate 9.88mg/(L·d)], and at 4000mL/d for the lipid production 3.86mg/(L·d)]. In this experiment, the lipid production in the optimal conditions is significantly higher than the control group. |
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| Keywords: | Chlorella pyrenoidosa photobioreactor environmental factors growth rate lipid accumulation |
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