| 牙鲆、石鲽和川鲽的线粒体16SrRNA基因片段的序列比较研究 |
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| 引用本文： | 孔晓瑜,刘亚军,喻子牛,庄志猛,武云飞. 牙鲆、石鲽和川鲽的线粒体16SrRNA基因片段的序列比较研究[J]. 中国海洋大学学报(自然科学版), 2001, 31(5): 713-722 |
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| 作者姓名： | 孔晓瑜 刘亚军 喻子牛 庄志猛 武云飞 |
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| 作者单位： | 青岛海洋大学水产学院 |
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| 基金项目： | 山东省自然科学基金(Y2000D04)和国家重点基础研究专项经费项目(G19990437)资助 |
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| 摘 要： | 以相应引物对牙鲆(Paralichthysolivaceus)和石鲽(K.areiusbicoloratus)的线粒体16SrRNA基因片段进行了PCR扩增,PCR产物经T载体连接之后进行了克隆、测序,均得到了590bp的碱基序列,并将其与GenBank中牙鲆线粒体全序列相应片段和川鲽(Platichthysflesus)相应片段进行比较,结果表明,川鲽和石鲽序列差异很小(核苷酸差异数为7,同源性为98.8%);而牙鲆与川鲽和石鲽的序列差异明显(核苷酸差异数为90～93,同源性约为84%),且大多数核苷酸变异位点集中在序列中部大约200bp的区域.
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| 关 键 词： | 牙鲆 石鲽 川鲽 16S rRNA基因 序列 中图法分类于Q178.53 Q953 文章编号 1001-1862(2001)05-713-05 |
| 文章编号： | 1001-1862(2001)05-713-05 |
| 修稿时间： | 2000-09-13 |
Comparative Study of Mitochondrial 16S rRNA Gene Fragments Among Paralichthysolivaceus Kareius bicoloratusand Platichthys flesus |
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| Kong Xiaoyu Liu Yajun Yu Ziniu Zhuang Zhimeng Wu Yunfei. Comparative Study of Mitochondrial 16S rRNA Gene Fragments Among Paralichthysolivaceus Kareius bicoloratusand Platichthys flesus[J]. Periodical of Ocean University of China, 2001, 31(5): 713-722 |
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| Authors: | Kong Xiaoyu Liu Yajun Yu Ziniu Zhuang Zhimeng Wu Yunfei |
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| Affiliation: | Kong Xiaoyu 1 Liu Yajun 1 Yu Ziniu 1 Zhuang Zhimeng 2 Wu Yunfei 1 |
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| Abstract: | Mitochondrial 16S rRNA gene fragments of flatfish Paralichthys olivaceus and Kareius bicoloratus were amplified via PCR, a nd the PCR products were ligated into T-vector, cloned and sequenced. 590bp nucl eotide sequences were retrieved for both species. The two sequences were aligned and compared with corresponding fragment of Platichthys.flesus and that of P.olivaceus mitochondrial complete genome. It's indicated that there is rela tively little sequence differences between P. flesuus and K. bicoloratus (homology 98.8%), but significant sequence differences exist between P.olivac eus and P. flesus, K. bicoloratus (homology around 84%), with most variabl e 200bp region in the middle. The sequences could be used for phylogenetic resea rch in flatfish species. |
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| Keywords: | Paralichthys olivaceus Kareius bicoloratus Plat ichthys flesus Mitochondrial 16S gene sequencing |
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