| 鲎血细胞提取物生物活性的初步研究 |
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| 引用本文： | 张海涛,梁景耀,翁云,祝其锋.鲎血细胞提取物生物活性的初步研究[J].海洋科学,2000,24(10):48-50. |
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| 作者姓名： | 张海涛 梁景耀 翁云 祝其锋 |
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| 作者单位： | 广东医学院生物化学与分子生物学研究所,湛江,524023 |
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| 基金项目： | 广东医学院青年基金资助项目!9804 |
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| 摘 要： | 采用酸抽提法和Ｓｅｐｈａｄｅｘ Ｇ－５０凝胶层析从鲎血细胞中分离出含有一低分子量组分的提取物，ＳＤＳ－ＰＡＧＥ显示一条区带。提取物可以抑制内毒素诱导的鲎试剂（ＴＡＬ）反应，抑制金色葡萄菌和大肠杆菌的生长，并发现２５ｍｇ／Ｌ的提取物对ＨＬ－６０细胞的生长有强烈的抑制作用。
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| 关 键 词： | 鲎血细胞 HL－60细胞 提取物 生物活性 酸抽提法 |
| 收稿时间： | 1999/7/14 0:00:00 |
| 修稿时间： | 6/1/2000 12:00:00 AM |
PURIFIED SUBSTANCE FROM HORSESHOE CRAB HEMOCYTES AND ITS BIOLOGICAL ACTIVITIES |
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| ZHANG Hai-tao,LIANG Jing-yao,WENG Yun,ZHU Qi-feng.PURIFIED SUBSTANCE FROM HORSESHOE CRAB HEMOCYTES AND ITS BIOLOGICAL ACTIVITIES[J].Marine Sciences,2000,24(10):48-50. |
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| Authors: | ZHANG Hai-tao LIANG Jing-yao WENG Yun ZHU Qi-feng |
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| Abstract: | Substance was purified from horseshoe crab blood cells by treated with acid and sephdex G 50 gel filtration.The SDS PAGE appeared one band after gelfiltration,the molecular of the substance is lower than 14 400D. It can inhibit the TAL test and the growth of G and G-, and strictly inhibit the HL 60 cells growing in 25 mg/L. |
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| Keywords: | Horseshoe crab hemocytes HL 60 cells Antimicrobial fraction Endotoxin |
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