The relationship between phytolith- and plant-water δO values in grasses

Information regarding climatic conditions during plant growth is preserved by the oxygen-isotope composition of biogenic silica (phytoliths) deposited in grasses. The O-isotope compositions of phytoliths and the plant water from which they precipitate are dependent on soil-water δ¹⁸O values, relative humidity, evapotranspiration rates, and temperature. Plant water and phytoliths from two grass species, Ammophila breviligulata (C₃) and Calamovilfa longifolia (C₄) at Pinery Provincial Park in southwestern Ontario, Canada, were examined to determine the variability in their δ¹⁸O values. Stem water was unfractionated from soil-water in oxygen isotopic composition and the δ¹⁸O values of stem silica provide a good proxy for the soil water available to roots during the growing season. Greater spatial and temporal variation in the δ¹⁸O values of water in the top 5 cm of the soil, and their enhanced sensitivity to evaporative ¹⁸O enrichment, are reflected in the generally higher δ¹⁸O values of water in the shallow roots and rhizomes of these grasses. Water within the sheath and lower and upper leaf tissues experiences continual evaporation, becoming progressively enriched in ¹⁸O as it moves towards the tip of the leaf. However, the water from which leaf silica precipitates has not acquired the extreme ¹⁸O enrichment predicted using steady-state models, or measured for midday or average daily leaf water. Possible explanations for this behaviour include preferential deposition of silica at night; the existence of a secluded water fraction within the leaf, which experiences smaller diurnal variations in isotopic composition than leaf water at sites of evaporation; kinetic isotope effects during rapid precipitation of leaf silica; and incomplete exchange between the oxygen in the silicic acid and the leaf water.