浒苔多糖对羟自由基的清除作用研究

研究确定了Fenten(水杨酸法)反应所需水杨酸、H2O2和Fe2+的浓度,并用水杨酸法对浒苔(Entero-morpha prolifera)水提多糖及其酸提多糖对羟自由基的清除能力进行了测定,同时,与合成抗氧化剂BHT和TBHQ进行对比。结果表明,浒苔水提多糖及酸提多糖对羟自由基均有一定的清除效果,且清除率与浓度存在剂量依赖关系,酸提多糖对羟自由基的清除能力大于水提多糖。在较高质量浓度(1.2 g/L)时酸提多糖对羟自由基的清除能力是水提多糖的2倍。

Study on the scavenging activity of polysaccharides from Ente romorpha prolifera to hydroxyl radical

The concentrations of salicylic acid, H₂O₂ and Fe²⁺ needed in Fenten reaction model were exa-mined firstly in this paper. Polysaccharides extracted by water (PW) and acid (PA) from Enteromorpha were prepared, and then their anti-oxidative activities were evaluated by radicals scavenging ratio to ·OH and compared with synthesized antioxidants BHT and TBHQ. The results showed that PW and PA all had certain activities to scavenge ·OH and in a dose dependent manner, and PA presented higher scavenging ratio to ·OH than PW. At the sample concentration of more than 1.2 g/L for PA, the scavenging ratio to ·OH doubled compared with PW. Therefore, it was suggested that polysaccharide by acid extraction from Enteromorpha should be a strong ·OH scavenger and thus has a bright future in food industry.