Preliminary genetic linkage map of the abalone Haliotis diversicolor Reeve |
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Authors: | Yaohua Shi Ximing Guo Zhifeng Gu Aimin Wang Yan Wang |
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Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, 266071, China;(2) Graduate University of Chinese Academy of Sciences, Beijing, 100049, China;(3) Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, Port Norris, NJ 08349, USA;(4) Zhejiang University, Hangzhou, Zhejiang, 310029, China;(5) Dalian Institute of Fisheries, Dalian, Liaonin, 116013, China; |
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Abstract: | Haliotis diversicolor Reeve is one of the most important mollusks cultured in South China. Preliminary genetic linkage maps were constructed with
amplified fragment length polymorphism (AFLP) markers. A total of 2 596 AFLP markers were obtained from 28 primer combinations
in two parents and 78 offsprings. Among them, 412 markers (15.9%) were polymorphic and segregated in the mapping family. Chi-square
tests showed that 151 (84.4%) markers segregated according to the expected 1:1 Mendelian ratio (P<0.05) in the female parent, and 200 (85.8%) in the male parent. For the female map, 179 markers were used for linkage analysis
and 90 markers were assigned to 17 linkage groups with an average interval length of 25.7 cm. For the male map, 233 markers
were used and 94 were mapped into 18 linkage groups, with an average interval of 25.0 cm. The estimated genome length was
2 773.0 cm for the female and 2 817.1 cm for the male map. The observed length of the linkage map was 1 875.2 cm and 1 896.5
cm for the female and male maps, respectively. When doublets were considered, the map length increased to 2 152.8 cm for the
female and 2 032.7 cm for the male map, corresponding to genome coverage of 77.6% and 72.2%, respectively. |
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