| Xp11.2区域YAC OAT1中表达顺序筛选 |
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| 引用本文： | 马洪明,曹立民,魏勇,柴建华.Xp11.2区域YAC OAT1中表达顺序筛选[J].中国海洋大学学报(自然科学版),2001,31(3):345-350. |
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| 作者姓名： | 马洪明 曹立民 魏勇 柴建华 |
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| 作者单位： | 1. 青岛海洋大学水产学院,
2. 复旦大学遗传学研究所 |
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| 基金项目： | 国家自然科学基金重点项目!(392 30 2 2 0 )资助 |
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| 摘 要： | 筛选 X染色体 Xp11.2区域的酵母人工染色体 ( YAC)克隆 OAT1所包含的表达序列 ,为该区域疾病相关基因的克隆探索方法。以 YAC克隆 OAT1为探针 ,与胎脑 c DNA文库的噬菌体原位裂解膜进行两轮杂交。所得的阳性克隆经过杂交鉴定剔除假阳性克隆和重复序列 ,并进行测序分析。获得了一个 YAC OAT1编码的表达顺序克隆。该克隆与鸟氨酸转氨酶 ( Ornithine Amino-transferase,OAT)基因同源
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| 关 键 词： | 酵母人工染色体(YAC) cDNA文库 杂交 |
| 文章编号： | 1001-1862(2001)03-345-06 |
| 修稿时间： | 1999年12月20 |
Screening of Expressed Sequences of YAC OAT1 in Xp11.2 |
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| Ma Hongming,Cao Limin,Wei Yong,CHAI Jianhua.Screening of Expressed Sequences of YAC OAT1 in Xp11.2[J].Periodical of Ocean University of China,2001,31(3):345-350. |
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| Authors: | Ma Hongming Cao Limin Wei Yong CHAI Jianhua |
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| Institution: | Ma Hongming1 Cao Limin1 Wei Yong2 Chai Jianhua2 |
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| Abstract: | To screen expressed DNA sequences harbored by YAC OAT1, which locates on Xp11.2, and to explore better means to clone new disease genes in this region, YAC OAT1 DNA was used as a probe to hybridize with the in situ lysised plaque of a fetal brain cDNA library. A second cycle of hybridization was performed to enrich the positive clones. The enriched positive clones hybridized with the YAC OAT1 and human genome DNA to eliminate the false ones or the ones that contain repetitive sequences. One expressed single copy sequence clone of YAC OAT1 was obtained. The sequencing analysis of the clone showed homology to the Human Ornithine Aminotransferase (OAT) gene. |
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| Keywords: | yeast artificial chromosome (YAC) cDNA library hybridization |
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