The scavenging of free radical and oxygen species activities and hydration capacity of collagen hydrolysates from walleye pollock (Theragra chalcogramma) skin |
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Authors: | Yongliang Zhuang Bafang Li Xue Zhao |
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Institution: | (1) College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, P. R. China |
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Abstract: | Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH
was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical
and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl
radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native
antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results
showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding
capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries. |
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Keywords: | walleye pollock collagen free radical-scavenging activity water-holding capacity |
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