Interactions of marine plankton with transuranic elements. II. Influence of dissolved organic compounds on americium and plutonium accumulation in a diatom

To assess the significance of naturally occurring dissolved organic matter (DOM) on complexation of transuranic elements in seawater, a series of bioassay experiments was conducted in which the effect of DOM on the accumulation of ²⁴¹ Am, ²³⁷Pu (III–IV), and ²³⁷Pu (V–VI) by the marine diatom Thalassiosira pseudonana was measured. EDTA at 0.3μM complexed both metals substantially, resulting in reduced radio-isotope uptake by the diatom; the greatest effect was on Pu (III–IV). In contrast, there was no apparent complexation of either element by equimolar concentrations of marine fulvic (MFA) or humic acids (MHA), naturally occurring photooxidizable DOM (uncharacterized), or diatom exudates, as none of these materials reduced isotope uptake; on the contrary, there were indications that some of this DOM enhanced transuranic bioaccumulation in the diatom slightly. Subsequent experiments showed this enhancement was probably due to complexation of transition metals by the DOM, leading to fewer ambient ions ‘competing’ for binding sites on the cells; ²⁴¹ Am uptake rates were negatively correlated (r =? 0.846, P < .01) with Σ ASV-labile Cu + Zn + Cd + Pb. These experiments suggest that naturally occurring DOM may not appreciably complex Am or Pu or greatly affect their bioavailability in the sea.