Occurrence of two distinct types of tissue inhibitors of metallo-proteinases-2 in Fugu rubripes |
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Authors: | Yoshihiro Yokoyama Hiroshi Tsukamoto Tohru Suzuki Shohshi Mizuta and Reiji Yoshinaka |
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Institution: | (1) Faculty of Biotechnology, Fukui Prefectural University, Japan;(2) Graduate School of Agricultural Science, Tohoku University, Japan |
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Abstract: | In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fish Fugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAs are composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteine residues, which might form six disulfide bonds as in other animals’ TIMP-2s. Reverse-transcribed
polymerase chain reaction analysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expression patterns. These findings suggest that
the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues. |
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Keywords: | Fugu rubripes TIMP-2 tissue inhibitor of metalloproteinase |
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