Spatial and temporal variations in free polyamine distributions in Uranouchi Inlet, Japan |
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Authors: | Naoyoshi Nishibori Yukihiko Matuyama Takushi Uchida Takamitu Moriyama Yoshihiko Ogita Masumi Oda Hitoshi Hirota |
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Institution: | a Shikoku University Junior College, Ojin, Tokushima 771-1192, Japan;b National Research Institute of Fisheries and Environment of Inland Sea, Ohno, Saeki, Hiroshima 739-0452, Japan;c Kochi Prefectural Fisheries Center, Susaki, Kochi 785-0167, Japan |
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Abstract: | Polyamines are an essential component of living organisms that influence normal cell growth and development. Despite the possible importance of polyamines for marine microbial ecology, concentrations of polyamines in seawater are not well known.Using HPLC analysis with a cation exchange column and OPA reagent, we determined polyamine concentrations in seawater without carrying out desalting, concentration or derivatization steps prior to chromatography.In seawater collected from Uranouchi Inlet, putrescine and spermidine were the predominant polyamines, ranging from undetectable (N.D.) to 4.4 nM and N.D. to 2.8 nM, respectively. Though other polyamines were detected, the frequency of detection was much lower than that of putrescine and spermidine; concentrations ranged from N.D. to 0.9 nM for cadaverine, and N.D. to 3.2 nM for norspermidine. Norspermine and spermine were detected in only a few samples; their concentrations varied from N.D. to 3.2 nM and N.D. to 0.5 nM, respectively. These concentrations were higher in the summer. |
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Keywords: | Coastal seawater Water chemistry Polyamine HPLC Cation exchange resin Uranouchi Inlet |
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