Morphological and toxicity characteristics of Pseudo-nitzschia pungens strain PP0201-01 isolated from the East China Sea |
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| Authors: | Li Aifeng Yu Rencheng Wang Yunfeng Yan Tian and Zhou Mingjiang |
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| Institution: | (1) Key Laboratory of Marine Ecology and Environment Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Graduate School, Chinese Academy of Sciences, 100039 Beijing, China |
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| Abstract: | The morphology and toxicity ofPseudo-nitzschia strain PP0201-01, isolated near the estuary of Changjiang (Yangtze) River in Year 2002 and tentatively identified asPseudo-nitzschia pungens, were studied. The strain was identified morphologically asP. pungens using scanning electronic microscope (SEM). The analytical results of LC-PDA (liquid chromatography with photodiode array
detector), LC-MS (liquid chromatography with mass detector), and the toxicity data from mouse bioassay, showed that no domoic
acid was produced by the strain. However, the mechanism of domoic acid production byPseudo-nitzschia species is complicated. More works are needed to assess the toxic species and to evaluate the potential risk of amnesic shellfish
poisoning (ASP) in China.
Supported by the projects of Ministry of Science and Technology (No. 2001CB409704 and No. 2001BA804A20), and the innovation
project of the Institute of Oceanology, CAS (No. 200223107). |
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| Keywords: | domoic acid Pseudo-nitzschia pungens scanning electronic microscope (SEM) mouse bioassay LC-UV LC-MS |
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