中国对虾微卫星DNA的筛选

于2000年2月以中国科学院海洋研究所水族楼暂养的中国虾对材料，采用常规方法从尾部肌肉中提取DNA，构建小片段部分基因组文章，采用人工设计合成的（CT）7，（AT）7重复征段作引物，利用RCR法对中国对虾小片段部分基因组文库进行筛,一实验首冼 对中国对虾中获得31个微卫星序列，分别分析于18个阳性重组克隆中，其中perfect共23个，占74％，imperfect2个，占7％，compound perfect1个，占3％，compound imperfect5个，占16％，结果还表明，在中国对虾基因组DNA中，（CT）n,(AT)n)形式的微卫星序列的含量非常丰富。

ISOLATING MICROSATELLITE DNA OF CHINESE SHRIMP PENAEUS CHINENSIS

Thirty-one microsatellites of the Chinese shrimp Penaeus chinensis were obtained through PCR screening small size fractionated libraries of P. chinensis with simple tandem repeats primers (AT)n and (CT)n. The bacterial suspensions were used in PCR. A high temperature of 95℃ was used for breaking the bacteria. After screening,recombinant positive clones containing microsatellites were sequenced. Among the 31 microsatellites in 18 positive recombinant positive clones are 23 perfect ones (74%),2 imperfect (7%),1 compound perfect (3%),and 5 compound imperfect (16%). The experiment shows that microsatellite sequences characterized by (AT)n and (CT)n abundant in genomic DNA of Chinese shrimp. Primers can be designed according to these microsatellite franking sequences and used in genetic analysis.