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Development of a real-time PCR method for Thalassiosira rotula rapid detection
引用本文:HE Shanying,YU Zhigang,MI Tiezhu. Development of a real-time PCR method for Thalassiosira rotula rapid detection[J]. 海洋学报(英文版), 2007, 26(2): 133-139
作者姓名:HE Shanying  YU Zhigang  MI Tiezhu
作者单位:HE Shanying1,YU Zhigang1,MI Tiezhu21. College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266003,China2. College of Environmental Science and Engineering,Ocean University of China,Qingdao 266003,China
基金项目:国家自然科学基金 , 国家高技术研究发展计划(863计划)
摘    要:Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.

关 键 词:圆海链藻 赤潮 快速检测 实时PCR法 18S rDNA
收稿时间:2006-03-07
修稿时间:2006-11-01

Development of a real-time PCR method for Thalassiosira rotula rapid detection
HE Shanying,YU Zhigang and MI Tiezhu. Development of a real-time PCR method for Thalassiosira rotula rapid detection[J]. Acta Oceanologica Sinica, 2007, 26(2): 133-139
Authors:HE Shanying  YU Zhigang  MI Tiezhu
Affiliation:1.College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China2.College of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, China
Abstract:Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.
Keywords:red tide   Thalassiosira rotula    fluorescent quantitative PCR   18S rDNA
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