Application of real time PCR determination to assess interanimal variabilities in CYP1A induction in the European flounder (Platichthys flesus) |
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Authors: | Dixon T J Taggart J B George S G |
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Institution: | Institute of Aquaculture, University of Stirling, Scotland, UK. |
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Abstract: | In both laboratory experiments and field investigations with fish a large interanimal variability in CYP1A expression has been observed which may be attributed to variations in environmental inducer exposure and/or inducer response. We are carrying out laboratory investigations to assess the contribution of a potential genetic component in inducer response of flounder (Platichthy sflesus) CYP1A to PCB exposure and in this paper we report development of a sensitive quantitative RT-PCR procedure (real time PCR) where accumulation of the intercalated dye SYBR Green I is followed during cycling. Preliminary experiments using this procedure with artificially reared Arochlor 1254-treated flounders showed large interanimal differences in response for a single family group indicating that variability does have a genetic basis. |
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