温度对绿海龟心、肝、肾功能生化指标的影响

分别在15,20,25和30 ℃海水中养殖绿海龟90 d,研究温度对绿海龟心、肝、肾等17项血液生化指标的影响。结果表明:心功能指标中,总胆固醇和甘油三酯均呈现先上升而后下降的趋势,25 ℃时含量最高,分别为3.83±0.30和0.60±0.08 mmol/L;高密度脂蛋白出现先降后升的变化;肌酸激酶含量随着温度的上升,呈下降趋势。肝功能指标中,白蛋白和总蛋白含量随着温度上升,先上升后下降,在25 ℃时达到最大值6.47±0.66和36.33±1.96 g/L;乳酸脱氢酶、白球比、碱性磷酸酶、谷草转氨酶、谷丙转氨酶和总胆红素都不随水温的变化而变化(P>0.05)。肾功能指标中,血尿酸和血肌酐含量随着温度上升而下降,血尿素氮没出现明显的变化(P>0.05)。综合温度对血液生化指标的影响规律,绿海龟在25 ℃左右储能物质消耗最少。

Effect of water temperature on the plasma biochemistry indexes of cardiac function,liver function and renal function from Green Turtles(Cheloniamydas)

Green Turtles(Cheloniamydas)were cultured at the water temperature 15, 20, 25 and 30 ℃ for 90 days to study the effects of different water temperature on 17 blood biochemical parameters. Results showed total cholesterol (CHOL) and triglyceride (TG) of cardiac function increased first and then decreased with the increase of water temperature and reached the maximum at 25 ℃, with the values of 3.83±0.30 mmol/L and 0.60±0.08 mmol/L respectively. High density lipoprotein (UHDL) tended to decrease first and then increase. The concentration of creatine kinase decreased with the water temperature increased. Of the liver function, albumin (ALB) and total protein (TP) increased first and then decreased with the temperature rise and reached the maximum at 25 ℃ with the values of 6.47±0.66 g/L and 36.33±1.96 g/L. Other index such as A/G, lactate dehydrogenase (LDH), alkaline phosphatase (ALP), aspertate aminotransferase (AST), alanine aminotransferase (ALT) and total bilirubin (TBIL) showed no significant changes while the water temperature raised (P>0.05). For the index of renal function, the concentration of blood uric (URIC) and creatinine (CREA) decreased while the water temperature increased and the concentration of urea nitrogen (BUN) had no significant change (P>0.05). Integrating the effect rule of water temperature on the plasma biochemistry, green turtles cultured at the water temperature 25 ℃ had a low storage material consumption.