| Isozymes Analysis of the Golden Cuttlefish Sepia esculenta（Cephalopoda ： Sepiidae） |
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| 摘 要： |
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| 关 键 词： | 墨鱼 同工酶分析 淀粉凝胶电泳技术 眼睛 肝脏 |
| 收稿时间： | 26 March 2003 |
| 修稿时间： | 17 December 2003 |
Isozymes analysis of the golden cuttlefish <Emphasis Type="Italic">Sepia esculenta</Emphasis> (Cephalopoda: Sepiidae) |
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| Authors: | Zheng Xiaodong Zhao Jianmin Xiao Shu Wang Rucai Wang Shidang Zhou Weiwu |
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| Institution: | (1) The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 266003 Qingdao, P.R.China;(2) Wendeng Fishery Technical Extension Station, 264400 Weihai, P.R.China |
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| Abstract: | Thirty-nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP,
IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram.
They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good
for some special enzymes, such as LDH, EST, MPI, etc. Twenty-six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao
sample (P<0.99). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017, while
those of the Rizhao sample are 0.023 and 0.025 respectively. |
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| Keywords: | cuttlefish Sepia esculenta isozyme electrophoresis genetic diversity |
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