FATTY ACIDS PROFILE IN A HIGH CELL DENSITY CULTURE OF ARACHIDONIC ACID-RICH PARIETOCHLORIS INCISA (TREBOUXIOPHYCEAE, CHLOROPHYTA) EXPOSED TO HIGH PFD |
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摘 要: |
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关 键 词: | 相位 脂肪酸 细胞 密度 对数 花生四烯酸 海藻 |
收稿时间: | 26 July 2000 |
修稿时间: | 21 March 2001 |
Fatty acids profile in a high cell density culture of arachidonic acid-richParietochloris incisa (Trebouxiophyceae,chlorophyta) exposed to high PFD |
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Authors: | Liu Jian-guo Zvi Cohen Amos Richmond |
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Institution: | (1) Research and Development Center of Morine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Microalgal Biotechnology, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Israel |
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Abstract: | The changes in arachidonic acid (AA) and fatty acids profiles along the growth curve ofParietochloris incisa, a coccoid snow green alga, were studied in a 2.8 cm light-path flat photobiorcactor, exposed to strong photon flux density
PFD, 2400 μEmol/(m2·s)]. Sixteen fatty acids were identified by gas chromatography showing that AA was the dominant fatty acid (33%–41%) followed
by linoleic acid (17%–21%). AA content was closely investigated with respect to total fatty acids (TFA), ash free dry weight
(AFDW) of cell mass as well as total culture content. These parameters were influenced significantly in a similar manner by
culture growth phase, i.e., slightly decreasing in the lag period, gradually increasing in the logarithmic phase, becoming
maximal at the early stationary phase, starting to decrease at the late stationary phase, sharply dropping at the decline
phase. The increase in AA per culture volume during the logarithmic phase was not only associated with the increase in AFDW
but also connected with a corresponding increase in AA/TFA, TFA/AFDW as well as AA/AFDW. The sharp decrease in AA content
of the culture during the decline phase was mainly due to the decrease in AA/TFA, TFA/AFDW and AA/AFDW, although AFDW declined
only a small extent. Maximal AA concentration, obtained at the early stationary phase, was 900 mg/L culture volume, and the
average daily net increase of AA during 9 days logarithmic growth was 1.7 g/(m2·day). Therefore, harvesting prior to the decline phase in a batch culture, or at steady state in continuous culture mode
seems best for high AA production. The latter possibility was also further confirmed by continuous culture with 5 gradients
of harvesting rate.
Contribution No. 4138 from the Institute of Oceanology, Chinese Academy of Sciences.
Project 39970575 supported by NSFC and A/2786-2 supported by International Foundation for Sciences (IFS). |
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Keywords: | arachidonic acid Parietochloris incisa growth high cell density culture |
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