南极菲尔德斯半岛土壤微生物在富集传代培养中对菲的响应

为揭示富集传代培养过程中南极多环芳烃降解菌群的演替规律,以菲为唯一碳源和能源对南极菲尔德斯半岛不同地理位置的6份土壤样品进行了富集和连续传代培养,并采用高通量测序技术分析了富集传代培养过程中菲降解菌群的群落结构及生物多样性.Alpha多样性分析结果表明,在富集培养阶段南极菲降解菌的物种丰富度显著高于传代培养阶段(P＜0...

RESPONSES OF SOIL MICROORGANISMS TO PHENANTHRENE IN ENRICHMENT CULTURES AND SUBCULTURES FROM FILDES PENINSULA, ANTARCTICA

To reveal the succession of the Antarctic polycyclic aromatic hydrocarbons (PAHs)-consuming bacterial communities, phenanthrene was utilized as the sole source of carbon and energy to enrich and subculture PAHs-degrading bacteria from soil samples at six different geographical stations in Fildes Peninsula, Antarctica. The bacterial community structures and biodiversity of the phenanthrene-degrading bacteria in enrichment culture and subcultures were analyzed by high-throughput amplified sequencing. Results of alpha diversity showed that the enrichment culture exhibited significantly higher species richness than those of the subcultures (P<0.05), and the species richness showed no significant difference among the subcultures (P>0.05). More importantly, results of beta diversity showed that the structure of phenanthrene-degrading bacterial consortia exhibited significant difference between the enrichment culture and the subcultures (P<0.05). A large number of microorganisms had high abundance in the enrichment culture stage, but they were eliminated in the subculture stage because they could not adapt to the laboratory culture conditions or could not use phenanthrene. In contrast, the structure of phenanthrene-degrading bacterial consortia exhibited no significant difference among different subcultures (P>0.05). The genera Pseudomonas, Sphingobium, Variovorax, Methylotenera, and Alcaligenes were the main phenanthrene-degraders, and they were stabilized dynamically in subcultures. In addition, Pseudomonas, Sphingobium, and Variovorax played a major role in maintaining dynamic balance in phenanthrene-degrading bacterial consortia. This study improved the understanding of degradation potential of persistent organic pollutants and the dynamics of the key species in Antarctic soil, and provided new insights into the exploitation and utilization of Antarctic microbial resources, especially for environmental protection.