温度、盐度和两种麻醉剂对大泷六线鱼幼鱼耗氧率、排氨率的影响

采用静水呼吸室法,研究了温度、盐度和两种麻醉剂(丁香油、MS-222)对体质量为(6.44±0.59)g的大泷六线鱼幼鱼耗氧率和排氨率的影响.实验分别设置了5个温度梯度(8℃、12℃、16℃、20℃、24℃),5个盐度梯度(15‰、20‰、25‰、30‰、35‰),6个丁香油浓度梯度(0、8、16、24、32、40 m...

Influence of temperature, salinity, and anesthetics on the oxygen consumption and ammonia excretion rates in fat greenling (Hexagrammos otakii) juveniles

The effects of temperature,salinity,and anesthetics(clove oil and MS-222)on the oxygen consumption and ammonia excretion rates of fat greenling(Hexagrammos otakii)juveniles,with a body weight of 6.44±0.59 g,was evaluated using the standing water respiratory chamber method.Temperature(8℃,12℃,16℃,20℃,and 24℃),salinity(15‰,20‰,25‰,30‰,and 35‰),clove oil(0,8,16,24,32,and 40 mg/L),and MS-222 treatments(0,10,20,30,40 mg/L)were evaluated.The results showed that temperature,salinity,and anesthetics have significant effects on the oxygen consumption and ammonia excretion rates.At 8℃,the oxygen consumption and ammonia excretion rates were lowest,increased with an increase of temperature,peaked at 20℃,and decreased at 24℃.At 30‰salinity,the oxygen consumption and ammonia excretion rates were lowest and increased with an increase or decrease in salinity.Both oxygen consumption and ammonia excretion rates were reduced effectively by clove oil or MS-222 and clove oil was more effective.The optimal concentrations for clove oil and MS-222 for the H.otakii juveniles to survive transportation were 24 mg/L and 20 mg/L,respectively,at which the fish were in deep sedation and oxygen consumption and ammonia excretion rates were low.The O︰N ratio was in the range of 14.77~24.11,indicating that the juvenile fish obtained energy mainly from protein and fat under suitable conditions of temperature and salinity.According to this study,the respiratory and metabolic activity of the H.otakii juvenile was low under moderate temperatures,natural salinity,or optimal concentrations of anesthetics—of which clove oil was more effective than MS-222.The results provide theoretical support for the transportation of high quality H.otakii.