兼性厌氧海洋细菌Shewanella sp.N3B_R的分离鉴定及产电性能分析研究

本研究的目的是利用聚苯胺修饰双室微生物燃料电池(MFC),从海水养殖废水中分离筛选阳极海洋产电菌,并利用响应面Box-Behnken设计优化各产电因子,提高产电性能.从海水养殖池塘的废水和污泥中分离出一株兼性厌氧海洋菌株,利用16SrRNA基因序列和API20NE生化分析进行鉴定,采用扫描电镜进行菌株形态的观察.采用循...

ISOLATION AND IDENTIFICATION OF FACULTATIVE ANAEROBIC MARINE BACTERIUM SHEWANELLA SP. N3B_R AND ANALYSIS OF ITS ELECTRICAL PERFORMANCE IN A TWO-COMPARTMENT MICROBIAL FUEL CELL

A strain was isolated from wastewater and sludge in mariculture ponds and identified using 16S rRNA gene sequences and API 20NE biochemical analysis.The isolated strain was identified as Shewanella sp.N3B_R and was used as a biocatalyst on an anode in a dual-chamber microbial fuel cell(MFC),and the electrochemical performance was investigated.Electrochemical impedance spectroscopy(EIS)analysis revealed that modifying an anode with polyaniline could reduce the charge transfer resistance in the MFC,resulting in 3 and 10 times higher output voltage and power density,respectively,than the corresponding MFC with an unmodified carbon cloth electrode.Optimization was achieved using Box-Behnken design of response surface method,from which the optimum conditions were:temperature of 35℃,5 g/L of aniline,and 0.2 mol/L of NaCl in concentration,under which the maximum yield of power density obtained was 522.1 mW/m².