Germination and algal-free laboratory culture of widgeon grass,Ruppia maritima

A methodology for the isolation ofRuppia maritima L. into algal-free laboratory culture is described. Seeds were surface sterilized with 96% ethyl alcohol of pH 2, kept for several months in cold storage at 7°C, and subsequently transferred to 25°C to induce germination. Basic culture conditions are discussed and a vessel of easy maintenance and sterilization forRuppia culture in ground glass sediments is presented. Minor modifications of this methodology might permit contaminant-free laboratory cultures of other estuarine and marine spermatophytes.