高浓度小新月菱形藻保存方法的研究

对小新月菱形藻(Nitzchia closterium f.minutissima)的保存方法进行了研究,试验证明,小新月菱形藻用不同的方法保存3个月,其最高存活率分别为:(1)在20℃下,15.0%的SAM8组为76.4%,1.5%的抗生素组为54.6%,0.1%的防腐剂组为45.3%,对照组仅为35.4%.(2)在0℃下,10.0%的SAM8组为98.4%,1.5%的抗生素组为78.8%,0.1%的防腐剂组为63.1%,低于对照组(65.5%).(3)-30℃下,10.0%的DMSO组为84.6%,10.0%的甘油组为83.1%,对照组为46.0%.为微藻的浓缩保存提供了新的方法.

Studies on the methods for storing Nitzschia closterium f.minutissima

This study deals with the storage of Nitzschiu closterium f.minutissima.After three months' storage,the following results have been carried out:(1)When t=20℃,the viability rate of N.closterium f.minutissirna was 76.4% in 15% SAM₈ group,54.6 % in 1.5% antibiotic group,45.3% in 0.1% antiseptic group,and 35.4% in control group.(2) When t=0C,the viability rate of N.closterium f.mrnutrssrrrra was 98.4% in 10% SAMa group,78.8% in 1.50% antibiotic group,63.1% in 0.1% antiseptic group,and 65.5% in control group.(3) When t=-30℃,the viability rate of N.closterium f.minutissima was 84.6% in 10% DMSO group,83.1% in 10% glycerine group,and 46.0% in control group.