Isolation and characterization of 45 Polymorphie microsatellite loci of turbot (Scophthalmus maximus) and cross-species amplification |
| |
Authors: | Shiying Hou Aijun Ma Xin��an Wang Zhihui Huang Baogui Xue Zhi Yang Jiangbo Qu |
| |
Institution: | 1Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology,Qingdao 266071,China 2Yantian Tianyuan Aquatic Limited Corporation,Yantai 264003,China 3Rushan Fisheries Technical Extension Station,Rushan 264500,China |
| |
Abstract: | Turbot (Scophthalmus maximus) is a flatfish species commercially important for aquaculture. In this study, we generated a microsatellite-enriched genomic
DNA library for Scophthalmus maximus, and then isolated and characterized 45 microsatellite loci by genotyping 30 individuals. The observed number of alleles
ranged from 2 to 19 with an average of 6.24, while the effective number of alleles ranged from 1.30 to 11.11 with an average
of 3.66. The expected heterozygosities varied from 0.235 to 0.925 4 and Polymorphie information content ranged from 0.2044
to 0.903 3, with an average of 0.622. Twelve loci deviated significantly from Hardy-Weinberg equilibrium, and no significant
linkage disequilibrium was observed between any pair of loci after Bonferroni correction. In cross-species amplification,
five flatfish species (Paralichthys lethostigma, Verasper moseri, platichthys stellatus, Hippoglossoides dubius and Cynoglossus semilaevis) showed at least one Polymorphie locus. These Polymorphie microsatellite loci should prove useful for Population analysis
of turbot and other related species. |
| |
Keywords: | |
本文献已被 CNKI SpringerLink 等数据库收录! |
|