土壤中五氯酚的快速测定

针对五氯酚(PCP)污染土壤,根据NaOH与五氯酚反应生成五氯酚钠的原理,将不易溶于水的PCP与碱液反应生成易溶于水的盐类,从而达到从土壤中提取五氯酚的目的。用紫外分光光度法定量测定五氯酚含量,确定出最佳提取与分析条件。结果表明,PCP-Na合适的紫外吸收波长为319 nm;在300 r/min振荡时,最佳提取条件为提取液pH>12.5、土液比为1∶8(g/mL)、提取时间为20 min。在此条件下,加标回收率为91.2%~116.7%,最低检测限为2μg/mL。方法快速、简便,适合于农田土壤中五氯酚的现场检测。

Rapid Determination of Pentachlorophenol in Soil Samples

Based on the principle of the reaction of NaOH with pentachlorophenol(PCP) and formation of PCP-Na,water-insoluble of PCP is transformed into water-soluble salts(PCP-Na) to realize the extraction of PCP from the soils.Ultraviolet spectrophotometric method was adopted to determine PCP at 319 nm.The extraction condition for PCP was optimized with the stirring rate of 300 r/min,extraction solution at pH>12.5,the ratio of soil/liquid=1/8(g/mL) and extraction time of 20 min.The sensitivity of the method is 2 μg/mL for PCP with recovery of 91.2%~108.1%.The method has been applied to the analysis of PCP in farmland soils with advantages of high efficiency and simple operation.