口服免疫药物后中国对虾某些血淋巴因子的测定及方法研究

于１９９１年４－９月，以海捕中国对虾亲虾及养殖中国对虾为材料，运用单向免疫扩散（ＳＲＩＤ）方法测定对虾血淋巴免疫因子，并采用经改进后的Ｈｏｒｏｗｉｔｚ等人的方法测定对虾体内的酚氧化酶（ＰＯ）活性，目的在于运用该两种方法对口服不同免疫药物的养殖对虾的血淋巴免疫因子及ＰＯ活力进行测定。对亲虾和养殖对虾口服不同免疫药物前后的测定结果表明，几种情况下的对虾血淋巴中均存在类似ＩｇＭ的因子，血细胞中存在酚氧化

MEASURING METHODS AND VARIATIONS OF SOME HAEMOLYMPH FACTORS IN PENAEUS CHINENSIS AFTER THEIR ORAL INGESTION OF IMMUNO DRUGS

From April to September, 1991, the single radial immunodiffusion (SRID) method was applied to determine the haemolymph factors in wild parent prawnsand cultured ones, including deseased ones, the phenoloxidase (PO) activity wasmeasured and its features studied after immuno drugs were orally ingested by theprawns. The results are given below.1. IgM-like substances existed in the haemolymph (which was most probablythe primitive form of IgM in mammals); PO existed in haemocytes in the form ofproenzyme and also exhibited activity in the normal serum of the prawns.2. After three kinds of natural drugs (extracted from land and marine plants)were taken orally by the cultured prawns, the content of haemolymph factors increased and PO activity decreased. The immune system of P. chincnsis could apparentlybe activated by two of those oral immuno drugs.3. SRID determination of IgM-like substances and measurement of PO activitymay be used as references to compare the immuno function of prawns.