The efficiency of kerosene flotation for extraction
of chironomid head capsules from lake sediments samples |
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Authors: | Nicolas Rolland Isabelle Larocque |
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Institution: | (1) Institut National de la Recherche Scientifique (INRS) Centre Eau, Terre et Environnement (ETE), 490, de la Couronne , Québec, (Qc), Canada, G1K 9A9 |
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Abstract: | Kerosene, a grade mineral oil, is commonly used to extract beetles from sediment. Here, the use of kerosene to extract chironomid
head capsules was tested on 10 samples from sediment of different lakes, with different organic matter content as measured
by loss on ignition, and estimated ages. Our results revealed that this flotation tool is very effective in extracting either
full or half chironomid head capsules. The mean extraction efficiency was 89.3 ± 8.0% with an estimated relative abundance
error ranging from −1% to 1% for 46 of the 57 identified taxa. Larger chironomids (400–500 μm width), which are often full
of sediment particles, have the highest relative abundance error, with a maximum of 4.3% for Corynocera oliveri-type. A canonical correspondence analysis showed that, despite this small bias, samples retrieved with the kerosene flotation
do not differ from the whole sample assemblages. These results give us confidence in the use of this flotation technique for
chironomid sample preparation. |
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Keywords: | Chironomid Kerosene Paraffin Flotation Extraction Transfer functions |
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