| 凡纳滨对虾EST-SSR标记的筛选及遗传多态性检测 |
| |
| 引用本文： | 张琼,李喜莲,薛淑雯,刘小林,黄皓,相建海.凡纳滨对虾EST-SSR标记的筛选及遗传多态性检测[J].海洋学报,2011,33(1):121-126. |
| |
| 作者姓名： | 张琼 李喜莲 薛淑雯 刘小林 黄皓 相建海 |
| |
| 作者单位： | 1.西北农林科技大学 动物科技学院 农业分子生物学陕西省重点实验室, 陕西 杨凌 712100 |
| |
| 基金项目： | 国家"十一五"八六三"重点项目(2006AA10A406);中国科学院实验海洋生物学开放课题。 |
| |
| 摘 要： | 为了研究我国海南地区某凡纳滨对虾(Litopenaeus vannamei)繁育群体的种质资源多样性,选取14个比利时野生对虾的EST微卫星标记,以240个中国人工选育的凡纳滨对虾个体为研究对象,检测这些标记在该养殖群体遗传多样性评估中的可行性,结果表明,有7个引物可扩增出清晰条带,其中5个引物(CNM-MG 345,...
|
| 关 键 词： | 凡纳滨对虾 EST-SSR 遗传多样性 |
| 收稿时间： | 3/4/2010 12:00:00 AM |
Screening and polymorphism of EST-microsatellite markers in Litopenaeus vannamei |
| |
| ZHANG Qiong,LI Xi-lian,XUE Shu-wen,LIU Xiao-lin,HUANG Hao and XIANG Jian-hai.Screening and polymorphism of EST-microsatellite markers in Litopenaeus vannamei[J].Acta Oceanologica Sinica (in Chinese),2011,33(1):121-126. |
| |
| Authors: | ZHANG Qiong LI Xi-lian XUE Shu-wen LIU Xiao-lin HUANG Hao and XIANG Jian-hai |
| |
| Institution: | 1.Key Laboratory of Shaanxi Province for Molecular Biology for Agriculture, College of Animal Sciences and Technology, Northwest Agricalture & Forest University, Yangling 712100, China2.Nanjiang Marine Biotechnology Company Limited, Sanya 572000, China3.Open of Laboratory Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China |
| |
| Abstract: | Fourteen microsatellite DNA universal markers derived from Belgian wild shrimps expressed sequences tags (ESTs) were selected and evaluated. Stable banding pattern was shown by 7 EST-SSRs, and five of(CNM-MG 345, CNM-MG 390, CNM-MG 437, CNM-MG 444, CNM-MG 487) them were identified as the polymorphism EST-SSR, and subsequently used for genotyping of 240 cultured adult white shrimps sampled from Hainan Province. The characterization results, which revealed 2.400 0±0.547 7 alleles per locus, observed and expected heterozygosity levels ranged from 0.279 2 to 0.820 8 and from 0.377 9 to 0.601 1 respectively, showed the moderate polymorphisms at these loci. In addition, genetic difference between the Belgian wild population and Hainan cultured population was firstly found, which might caused by geographic isolation and selective breeding over the years. This study set The foundation for population genetics studies, function gene analysis and selective breeding was set in Hainan cultured Litopenaeus vannamei. |
| |
| Keywords: | Litopenaeus vannamei EST-SSR genetic diversity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋学报》浏览原始摘要信息 |
| 点击此处可从《海洋学报》下载免费的PDF全文 |
|
