| ISOLATION OF PLASMID FROM THE BLUE-GREEN ALGA SPIRULINA PLATENSIS |
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| 作者姓名： | 秦松 童顺 张培军 曾呈奎 |
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| 作者单位： | Experimental Marine Biology Laboratory，Institute of Oceanology，Academia Snica，Qingdao 266071，Experimental Marine Biology Laboratory，Institute of Oceanology，Academia Snica，Qingdao 266071，Experimental Marine Biology Laboratory，Institute of Oceanology，Academia Snica，Qingdao 266071，Experimental Marine Biology Laboratory，Institute of Oceanology，Academia Snica，Qingdao 266071 |
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| 基金项目： | This research was supported in part by The President of The Chinese Academy of Sciences Foundation |
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| 摘 要： | CCC plasmid was isolated from an economically important blue-green alga- Spirulina platensis (1.7×106 dalton from the S6 strain and 1.2×106 dalton from the F, strain) using a rapid method based on ultrasonic disruption of algal cells and alkaline removal of chromosomal DNA. The difference in the molecular weight of the OOC DNAs from the two strains differing in form suggests that plasmid may be related with the differentiation of algal form. This modified method, which does not use any lysozyme, is a quick and effective method of plasmid isolation, especially for filamentous blue-green algae.
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ISOLATION OF PLASMID FROM THE BLUE-GREEN ALGA SPIRULINA PLATENSIS |
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| QIN Song,TONG Shun ZHANG Peijunand C. K. TSENG.ISOLATION OF PLASMID FROM THE BLUE-GREEN ALGA SPIRULINA PLATENSIS[J].Chinese Journal of Oceanology and Limnology,1993(3). |
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| Authors: | QIN Song TONG Shun ZHANG Peijunand C K TSENG |
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| Abstract: | |
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| Keywords: | plasmid blue-green alga Spindina platensis |
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