Manganese-reducing Pseudomonas fluorescens-group bacteria control arsenic mobility in gold mining-contaminated groundwater

Previous studies show the importance of iron- and arsenate-reducing bacteria in mobilizing arsenic in groundwater. Here the authors present experimental evidence of arsenic mobilization in connection with bacterially mediated manganese reduction in groundwater affected by mining activities. Manganese-reducing Pseudomonas species were enriched, isolated and identified by 16S rRNA gene phylogeny from groundwater containing high co-dissolved arsenic (as As^III) and manganese. Enrichment cultures dissolved synthetic birnessite and hausmannite efficiently, but Mn reduction by isolates was reduced at the upper range of environmental levels of dissolved As^III. Results suggest either a self-limiting release of arsenic coupled to bacterial manganese reduction, in the absence of other electron donors like sulfide, or increased arsenic resistance conferred to Mn-reducing bacteria in consortia.