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The stabilisation of wet sediment cores by means of a polyethylene glycol/freeze-drying treatment for display and permanent storage
Authors:Per Hoffmann and Jürgen P?tzold
Institution:(1) Department of Physics, Indiana University-Purdue University, Indianapolis, IN, USA;(2) Laboratory of Physical and Structural Biology, NICHHD, National Institutes of Health, Bethesda, MD, USA;
Abstract:A reversible method is presented to transform waterlogged sediment cores into dry and stable specimens which can go on exhibition or into permanent storage, needing no special precautions. Wet sediment core segments are bath-impregnated with polyethylene glycol of molecular weight 3,350 (PEG 3,350) dissolved in water. The samples are then subjected to a freeze-drying process, during which the PEG forms a stabilising and bonding porous network structure in the capillary system of the sediment. Standard sediment cores at least 1 m long become strong enough to stand upright. Sediment types ranging from muddy deep-sea sediments to very coarse littoral Halimeda sand were successfully stabilised. For standard cores of 10-cm diameters split length-wise, impregnation times vary from 2 weeks for coarse sand to 6–10 weeks for more compacted sediments. With regard to the stability of the samples and the visual clarity of detail, best results were obtained by impregnation with 25 to 60% solutions of PEG 3,350, coarse sediments needing more PEG than finer ones. Colour changes are systematic – the processed samples are lighter in appearance and the contrasts are enhanced, but there is no serious shift in colour tones. The PEG can easily be re-dissolved and washed out of the sediments. Display specimens can thus be made available for research again.
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