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Minicistron from a gene of prawn white spot bacilliform virus (WSBV) and its expression
Authors:Zhang Xiaobo  Xu Xun and Yang Feng
Institution:1.School of Life Science, University of Science and Technology of China, Heifei 230026, China2.School of Life Science, University of Science and Technology of China, Heifei 230026, China;Key Laboratory of Marine Biotechnology, Third Institute of Dceanography, State Oceanic Administration, Xiamen 361005, China3.Key Laboratory of Marine Biotechnology, Third Institute of Dceanography, State Oceanic Administration, Xiamen 361005, China
Abstract:By the sequencing and analysis of genomic DNA and cDNA of WSBV, it was found that these existed a minicistnni in the leading sequence of p204 gene.The minicistron utilized rare oodons of genes from prawn and WSBV.The p204 gene could be expressed in Escherichia coli whether a miniciston exited in its leading sequence or not, but the amounts of the expressed products of them were different.The minicistron might be related to the expression of p204 gene.
Keywords:Prawn white spot bacilliform virus  minicistron  frequency of codon  expression
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