栉江珧第1卵裂抑制型雌核发育二倍体人工诱导的研究

利用6-二甲基氨基嘌呤(60 mg/L;6-DMAP)抑制第一卵裂,成功诱导出栉江珧(Atrina pectinata)雌核发育二倍体.研究结果表明在培养温度为24℃的条件下,受精后60 min用质量浓度为60 mg/L的6-DMAP处理栉江珧受精卵15 min进行雌核发育二倍体的诱导效果理想,D形幼虫发生率和诱导率分别为14.7%和22.7%.细胞学观察显示,6-DMAP阻止了纺锤体的形成和染色体的移动,导致一个融合的二倍性雌性原核的形成.本研究结果首次提供了栉江珧雌核发育二倍体的细胞学证据.

Artificial induction of gynogenetic diploids by suppression of the first cleavage in Atrina pectinata

The induction of gynogenesis diploids by suppression of the first cleavage in Atrina pectinata was attempted using the treatment of 6-dimethylaminopurine(60 rag/L; 6-DMAP). The results showed that the treatment of zygotes with 6-DMAP at 60 rain postinsemination for 15 min at water temperature of 24℃ produced more expected D-shaped larvae, and the rate of D-shaped larvae and level of diploids were 14. 7% and 22.7%, respectively. According to cytological observations, 6-DMAP disrupted the spindle at mi-tosis and inhibited chromosome movement, resulting in the formation of one diploid female nucleus. Cyto-logical evidence of the induction of gynogenetic diploids in A. Pectinata was demonstrated.