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Comparative sensitivity of harbour and grey seals to several environmental contaminants using in vitro exposure |
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| Authors: | MM Dufresne S Pillet S De Guise |
| Institution: | a Université du Québec à Montréal, Montréal, Québec, Canada H3C 3P8 b Institut National de la Recherche Scientifique - Institut Armand-Frappier, Laval, Québec, Canada H7V 1B7 c Fisheries and Oceans Canada, Maurice Lamontagne Institute, Mont-Joli, Québec, Canada G5H 3Z4 d Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, USA |
| Abstract: | In this study, we investigated the effects of cadmium chloride (CdCl2), mercury chloride (HgCl2), methylmercury chloride (CH3HgCl), and PCBs on lymphocyte proliferation in phocids. PBMCs isolated from harbour and grey seals were exposed in vitro to varying concentrations of contaminants. A reduction of viability occurred when cells were exposed to 10−4 M HgCl2 or CH3HgCl or to 50 ppm of Aroclor 1254. In both grey and harbour seals, T-lymphocyte proliferation was suppressed when their cells were incubated with 5 × 10−5 M CdCl2 or 10−4 M HgCl2. An inhibition of proliferation occurred with CH3HgCl from 10−6 M in grey seals and from 10−5 M in harbour seals. In grey seals, Aroclor 1254 reduced lymphocyte proliferation at 15 ppm. In both harbour and grey seals, CH3HgCl was ten times more immunotoxic that HgCl2. From IC50, chemicals were ranked in terms of toxicity as followed: CH3HgCl > CdCl2 > HgCl2 > Aroclor 1254. |
| Keywords: | Immunotoxicity Mercury Cadmium PCBs Harbour seals Grey seals |
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