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Preliminary Study on Applicability of Microsatellite DNA Primers from Parasite Protozoa Trypanosoma cruzi in Free-living Protozoa
作者姓名:ZHANGWenjing  YUYuhe  SHENYunfen  MIAOWei  FENGWeisong
作者单位:ZHANG Wenjing,YU Yuhe *,SHEN Yunfen,MIAO Wei,FENG Weisong Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,P.R. China
基金项目:the Frontier Science Projects Programme of the Institute of Hydrobiology,中国科学院资助项目
摘    要:1 Introduction Generallyknownasacodominantgeneticmarker ,microsatellitehasbeenwidelyusedinstudiesonpopu lationgenetics,high resolutiongenotyping ,genemap ping ,evolution ,linkageanalysis ,conservationbiology ,behaviouralecology ,relationsbetweenparasite…

关 键 词:原生动物  DNA分析  锥虫  多行性  水生生物
收稿时间:19 December 2003
修稿时间:24 March 2004

Preliminary study on applicability of microsatellite DNA primers from parasite protozoa <Emphasis Type="Italic">Trypanosoma cruzi</Emphasis> in free-living protozoa
ZHANGWenjing YUYuhe SHENYunfen MIAOWei FENGWeisong.Preliminary Study on Applicability of Microsatellite DNA Primers from Parasite Protozoa Trypanosoma cruzi in Free-living Protozoa[J].Journal of Ocean University of China,2004,3(1):80-84.
Authors:Zhang Wenjing  Yu Yuhe  Shen Yunfen  Miao Wei  Feng Weisong
Institution:Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China
Abstract:In this paper, we took the lead in studying on specificity of the microsatellite DNA loci and applicability of mi crosatellite DNA primers in protozoa. In order to study characters of microsatellites in free living protozoa, eight microsatellite loci primers developed from Trypanosoma cruzi (MCLE01, SCLE10, MCLE08, SCLE11, MCLF10, MCLG10, MCL03, MCL05) were employed to amplify microsatellite in four free living protozoa, including Bodo designis, Euglena gracilis FACHB848, Paramecium bruzise and Tetrahymena thermophila BF1. In the amplification systems of P. bruzise, four loci (SCLE10, SCLE11, MCLF10, MCL03) were amplified successfully, and four amplification fragments were in proper size. In genome of E. gracilis FACHB848, five of eight primers brought five clear amplification bands. In B. designis, three (No.4, 5 and 7) of eight loci produced clear and sharp products without stutter bands, whereas no bands appeared in T. thermophila BF1. Further, eight 300-500 bp amplification fragments were cloned and sequenced. Nevertheless, all sequenced products did not contain corresponding microsatellite sequence, although Bodo is in the same order and has the nearest phylogenetic relation with Trypanosoma among these four species. Thus, the microsatellite DNA primers can not be applied among order or more far taxa, and the specificity of microsatellite DNA is very high in protozoa. The results of this study will contribute to our understanding of microsatellite DNA in protozoa.
Keywords:protozoa  microsatellite DNA  amplification  applicability of primers
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