Gel- and ultramembrane filtration of aquatic humus: A comparison of the two methods

Fractionation is considered to be a necessary approach in order to obtain information about the complex composition of aquatic humus. Gel filtration has been used for this purpose for the last decade and recently ultramembrane filtration has been found useful. The two techniques are briefly described and results of a combined use of the two methods reported. The results show a poor correspondence, with regard to molecular size, between the two methods. Due to uncontrollable interaction between the gel used (Sephadex) and humus, the ultramembrane filtration technique is considered to be preferable for desalting and molecular size fractionation of aquatic humus.