The use of cotton blue stain to improve the efficiency of picking and identifying chironomid head capsules |
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Authors: | Isabelle Larocque-Tobler Florencia Oberli |
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Institution: | 1.Institute of Geography and Oeschger Center for Climate Change Research,University of Bern,Bern,Switzerland;2.INRS-ETE,Québec,Canada;3.Institute of Plant Sciences,University of Bern,Bern,Switzerland |
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Abstract: | Cotton blue was added to sediment samples at least 2 h before chironomid head capsules were picked under a binocular microscope
and mounted on slides for identification. The use of stain greatly increased the visibility of chironomid head capsules during
picking and enhanced the contrast of various parts of the head capsules (pores, ventromental plates, striations on ventromental
plates), which could aid identification. In the seven samples studied, there was no significant difference between the percentages
of taxa found in stained and unstained samples. The number of taxa were also similar in stained and unstained samples. This
method allowed samples to be picked faster. |
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Keywords: | |
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