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Study on tissue culture forGelidium seedling
Authors:Pei Lu-qing  Luo Qi-jun  Fei Zhi-qing  Ma Bin
Institution:(1) Zhejiang Fisheries College, 315010 Ningbo
Abstract:As seedling culture is a crucial factor for successful cultivation ofGelidium, the authors researched tissue culture technology for producing seedlings. The morphogeny and experimental ecology were observed and studied fully in 2–5 mm isolated tissue fragments. Regeneration, appearance of branching creepers and attaching structure and new erect seedlings production and development were studied. Fragments were sown on bamboo slice and vinylon rope. The seedlings were cultured 20–30 days indoor, then cultured in the sea, where the density of erect seedlings was 3–19 seedlings/cm2, growth rate was 3.84% day. The frond arising from seedlings directly was up to 10 cm per year. The ecological conditions for regenerated seedlings are similar to the natural ones. The regenerated seedlings are suitable for raft culture in various sea areas.
Keywords:Gelidium Lamx  fragment  tissue culture  seedling
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