温度和盐度对军曹鱼幼鱼生长与抗氧化酶活性的影响

在实验室条件下,采用两因素交叉分组的方法,初步研究了环境因子(温度和盐度)对军曹鱼幼鱼肌肉抗氧化酶比活力的影响。温度设3个水平(26、29、32),盐度设4个水平(11、19、27、35),两个因素按其各自水平共产生12种组合。军曹鱼幼鱼在玻璃钢水槽内养殖20d后取样测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPX)的比活力。结果表明:盐度因子诱导了军曹鱼的抗氧化应激反应,抗氧化酶活力随盐度降低而升高,SOD、CAT和GPX比活力分别从35时的17.74±3.38、19.44±2.66和13.70±2.99U/mg上升到11时的30.14±1.76、35.63±10.59和17.44±4.32U/mg;温度、盐度及因素间的交互作用对SOD和CAT比活力有显著影响(P<0.05);温度对GPX比活力无显著影响(P>0.05),而盐度及因素间的交互作用对GPX比活力影响显著(P<0.05)。

Effects of Temperature and Salinity on the Growth and Activities of Antioxidant Enzymes of Cobia (Rachycentron canadum) Juveniles

Two-factor-intersecting experiment was designed to evaluate the effects of temperature and salinity on the antioxidant enzymatic activities in the muscles of cobia (Rachycentron canadum) juveniles under the laboratory conditions. In this experiment three levels of water temperature(26, 29, 32) were set, and four levels of salinity(11, 19, 27, 35) were set respectively. Two factors engendered 12 combinations in all on each level. The cobia juveniles had been reared in the glass fiber tanks for 20 days. Then the fish samples were obtained randomly from each tank to examine the activities of SOD (Superoxide dismutase), CAT (Catalase) and GPX (Glutathione Peroxidase). The results showed that salinity induced adaptive responses such as increase in the antioxidant enzymatic activities, mainly those of SOD and CAT, as well as in GPX values. The activities of SOD,CAT and GPX increased from 17.74±3.38, 19.44±2.66 and 13.70±2.99 U/mg at 35 to 30.14±1.76, 35.63±10.59 (P<0.05) on the activities of SOD and CAT. Except temperature, the salinity and interaction all had significant effect (P<0.05) on the activity of GPX.