赤潮发生机理研究──海洋原甲藻的氮营养生理特征

于１９９１年１月－１９９１年１２月，以不同浓度的硝态氮（２０，６０，２００，６００μｍｏｌ／Ｌ）研究对赤潮生物海洋原甲藻的生长和生化组成的影响，及其营养生理特性，结果表明，在氮浓度为２００和６００μｍｏｌ／Ｌ时，表现光合作用强，细胞生长快，指数生长期长，叶绿素含量高，提示了由原甲藻爆发引起的赤潮与海洋富营养化有直接关系，结果还表明，海洋原甲藻可以累积蛋白质和碳水化合物，当外界氮源不能满足生长需要时

STUDY ON NITROGEN METABOLISM OF PROROCENIRUM MICANS WITH SPECIAL REFERENCE TO THE OCCURRENCE OF RED TIDE

The physiological aspcts of nitrogen nutrition of Prorocentrum micans were studied in the algal physiological section of the Institute of Oceanology from Jan.through Dec., 1991.Seawater was filtered, sterilized and modified by addition of KH2PO4`, FeC6H5O7 vitamin B, and K at 4mg /L, 0.5mg /L, l00ng /L and O.5Pg /L,respectively. The algae were cultured in modified seawater of different concentrations (20, 60, 200 and 600umol /L) of nitrate- Cultures Were grown under irradianee of ca.500pE /(m2.s) and temperature of 221-During the experiment, the growth, apparent photosynthesis, nitrate absorption rate, contents of chlorophyll a, crude protein and carbohydrates were measured periodically. The results were as follows. In all nitrate-nitrogen enriched culture media (20, 60, 200 and 600umol /L), longer exponential growth periods, higher contents of chlorophyll a and higher apparent photosynthetic rates were found, suggsting a positive corelation of eutrophication with dinoflagellates caused red tide- P micans absorbed more nitrate at night (16:00- 18:00) and On the contrary more carbohydrates were found in the cells at daytime. ms was because more carbohydrates were metabolized with nitrogen at night. Part of the proteins so formed was stored in the cell' In the case of nitrogen starvation, the exponential growth could be maintained up to 6 days by the consumption of stored proteins.