Chemical and biological characterization of particles by flow cytometry in the Krka estuary, Croatia

Flow cytometry was used to characterize suspended particles in the Krka estuary (Croatia). This technique allows a rapid characterization and quantification of individual particles; it is based on the simultaneous measurement of the light scatter and natural or induced fluorescence. The relative size of the particles was determined by light scatter signals and by a calibration with fluorescent beads.The determination of organic particles was carried out by staining with fluorescein isothiocyanate (FITC). The autofluorescence of photosynthetic pigments (chlorophyll and phycoerythrin) present in the phytoplankton population was measured. The carbohydrates (galactose and glucose-mannose) were stained by lectins conjugated to FITC. The abundance of organic coatings or mineral particles and detritus was obtained from the difference between the total number of particles stained by specific carbohydrate fluorochromes and living cells.Results show that the Krka estuary is characterized by the occurrence of very small particles. About 80% of suspended particles are organic, most of these particles being living cells. The only exception is the Guduca River, a small tributary.