Recovery of ammonium nitrogen by solvent extraction for the determination of relative 15N abundance in regeneration experiments |
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| Affiliation: | 1. RIKEN CLST-JEOL Collaboration Center, RIKEN, Yokohama, Kanagawa 230-0045, Japan;2. JEOL RESONANCE Inc., Musashino, Akishima, Tokyo 196-8558, Japan;1. Center for Ecosystem Science and Society (Ecoss) and Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA;2. Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA;3. Geography, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4 RJ, UK;1. Institute of General and Inorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent 100170, Uzbekistan;2. Materials and Structures Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori, Yokohama, Kanagawa 226-8503, Japan;3. Department of Natural and Mathematic Sciences, Turin Polytechnic University in Tashkent, Kichik Halqa Yo''li 17, Tashkent 100095, Uzbekistan;4. School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi''an 710055, PR China;5. Department of Textile Engineering, University of Uludağ, Gorukle, Bursa 16059, Turkey;1. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China;2. National Engineering Laboratory for Improving Quality of Arable Land, Beijing, 100081, China;3. Key Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Agricultural Water-Saving, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, 050021, China;4. Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar City, Heilongjiang Province 161005, China;5. College of Natural Resources and Environment, Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shanxi Province 712100, China;4. Land Reclamation International Graduate School, University of Alberta, Edmonton, Alberta T6G 2E3, Canada |
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| Abstract: | A new sample preparation method for 15N tracer measurement of ammonium regeneration in seawater is described. Ammonium nitrogen is incorporated into indophenol, extracted into dichloromethane and concentrated by evaporation. The isotopic ratio of the indophenol nitrogen is determined by emission spectroscopy. A small amount (0.2 μmol) of unlabeled nitrogen is introduced by reagents used during sample preparation. For samples of equal nitrogen content, the coefficient of variation is constant and the precision of the isotopic ratio determination is inversely related to the relative 15N abundance. Differences of 0.19 atom% 15N can be measured in 5.0 μM NH4+ samples of 5.0 atom% 15N (SD = 0.07, n = 3, p = 0.05). Interface from forms of dissolved organic nitrogen prevalent in marine environments are negligible. |
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