| 长心卡帕藻原生质体分离、培养研究 |
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| 引用本文： | 张思,刘翠,金月梅,池姗,唐贤明,陈傅晓,方诩,刘涛.长心卡帕藻原生质体分离、培养研究[J].海洋学报(英文版),2014,33(10):114-123. |
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| 作者姓名： | 张思 刘翠 金月梅 池姗 唐贤明 陈傅晓 方诩 刘涛 |
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| 作者单位： | 国家海洋环境预报中心, 北京100081, 中国;中国海洋大学海洋生命学院, 青岛266003, 中国;中国海洋大学海洋生命学院, 青岛266003, 中国;中国海洋大学海洋生命学院, 青岛266003, 中国;中国海洋大学海洋生命学院, 青岛266003, 中国;海南省水产研究所, 海口570204, 中国;海南省水产研究所, 海口570204, 中国;山东大学生命科学学院, 济南250100, 中国;中国海洋大学海洋生命学院, 青岛266003, 中国 |
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| 基金项目： | The National Science Foundation Project under contract No. 2007FY210500; the National Department Public Benefit Research Foundation of China under contract No. 200805075; the Province Science and Technology in the Guangdong Project under contract Nos 2010B060200010 and 2010B020201015; the Science Expenditure in the Hainan Project under contract No. 11-20410-0015; the National Natural Science Foundation of China under contract Nos 41206106 and 41222038. |
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| 摘 要： | In this study, protoplasts were successfully isolated from Kappaphycus alvarezii using snail enzymes, abalone enzymes and cellulase. The optimum enzymic ratio was fixed to be 20% of abalone enzyme, 12% of cellulase and the osmotic stabilizer was 2.0 mol/L glucose. The optimum enzymic hydrolysis conditions were found to be dark enzymolysis at 30°C continuing for 4.0 h. The resultant density and yield of protoplasts achieved 32.60×104 mL-1, 65.20×104 g-1 tissue for Kappaphycus alvarezii. Finally, under the temperature of 20°C, light intensity of 1 500–2 000 lx and photoperiod of 12 h/d, two developmental pathways were investigated:(1) callus-like cell mass and regenerated plantlet occurred on protoplast;(2) young shoots and calluslike cell mass occurred in tissue blocks after enzymolysis.
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| 关 键 词： | 原生质体 分离培养 渗透压稳定剂 愈伤组织 藻 纤维素酶 蜗牛酶 水解条件 |
| 收稿时间： | 2013/4/16 0:00:00 |
| 修稿时间： | 2013/11/12 0:00:00 |
Studies on the isolation and culture of protoplasts from Kappaphycus alvarezii |
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| Zhang Si,Liu Cui,Jin Yuemei,Chi Shan,Tang Xianming,Chen Fuxiao,Fang Xu and Liu Tao.Studies on the isolation and culture of protoplasts from Kappaphycus alvarezii[J].Acta Oceanologica Sinica,2014,33(10):114-123. |
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| Authors: | Zhang Si Liu Cui Jin Yuemei Chi Shan Tang Xianming Chen Fuxiao Fang Xu and Liu Tao |
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| Institution: | 1.National Marine Environmental Forecasting Center, Beijing 100081, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China2.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China3.Hainan Provincial Fisheries Research Institute, Haikou 570204, China4.School of Life Science, Shandong University, Jinan 250100, China |
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| Abstract: | In this study, protoplasts were successfully isolated from Kappaphycus alvarezii using snail enzymes, abalone enzymes and cellulase. The optimum enzymic ratio was fixed to be 20% of abalone enzyme, 12% of cellulase and the osmotic stabilizer was 2.0 mol/L glucose. The optimum enzymic hydrolysis conditions were found to be dark enzymolysis at 30℃ continuing for 4.0 h. The resultant density and yield of protoplasts achieved 32.60×104 mL-1, 65.20×104 g-1 tissue for Kappaphycus alvarezii. Finally, under the temperature of 20℃, light intensity of 1 500-2 000 lx and photoperiod of 12 h/d, two developmental pathways were investigated: (1) callus-like cell mass and regenerated plantlet occurred on protoplast; (2) young shoots and calluslike cell mass occurred in tissue blocks after enzymolysis. |
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| Keywords: | Kappaphycus alvarezii protoplast regenerated plantlet callus-like young shoots |
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