高盐胁迫对凡纳滨对虾(Litopenaeus vannamei)生长、代谢和抗氧化酶活力的影响

为分析高盐胁迫对凡纳滨对虾(Litopenaeus vannamei)生长、代谢和抗氧化酶活力的影响,实验以平均体重(3.0±0.4)g,平均体长(7.6±0.2)cm的凡纳滨对虾为实验材料,设置25(对照组)、35、45、55四个盐度梯度,分别于实验开始后0d、10d、20d、30d取样,检测对虾体重、体长、甘油三酯(TG)、总胆固醇(TC)和三磷酸腺苷(ATP)的含量以及苹果酸脱氢酶(MDH)、总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)活力,计算增重率、增长率、特定生长率和成活率。结果表明,随着盐度升高,凡纳滨对虾的增重率、增长率、特定生长率和存活率均逐渐降低, 55盐度组各生长指标均显著低于对照组(P<0.05);10d时TG含量随着盐度的增加逐渐降低,55盐度组显著低于对照组(P<0.05)。20d和30d时逐渐升高, 55盐度组显著高于对照组(P<0.05); 55盐度组的TC含量最高,且30d时显著高于其他组(P<0.05); 45盐度组、55盐度组MDH活力在10d时显著低于对照组(P<0.05),但20d和30d时显著高于对照组(...

EFFECT OF HIGH-SALINITY STREE ON GROWTH, METABOLISM, AND ANTIOXIDANT ENZYME ACTIVITIES OF LITOPENAEUS VANNAMEI

To analyze the effect of high-salinity stress on the growth, metabolism, and antioxidant enzyme activities of Litopenaeus vannamei, the shrimps in average body weight (3.0±0.4)g and average body length (7.6±0.2)cm were used as the experimental materials. Four salinity gradients of 25 (control group), 35, 45, and 55 were set up. Samples were taken at on Days 0, 10, 20, and 30. The body weight, body length, the contents of triglyceride (TG), total cholesterol (TC), and adenosine triphosphate (ATP), the activities of malate dehydrogenase (MDH), total superoxide dismutase (T-SOD), and catalase (CAT) were measured, and weight gain rate, body length growth rate, specific growth rate, and survival rate were calculated. The results show that body length growth rate, weight gain rate, specific growth rate, and survival rate of L. vannamei decreased gradually with the increase of salinity. The growth indexes of salinity 55 group were significantly lower than that of the control group (P<0.05). The TG content of salinity 55 group decreased gradually with the increase of salinity on Day 10, and was significantly lower than that of the control group (P<0.05). The TC content of salinity 55 group was higher than that of the control group (P<0.05). The MDH activities of salinity 45 and 55 groups were significantly lower than that of the control group on Day 10 (P<0.05), but significantly higher than that of the control group on Days 20 and 30 (P<0.05). ATP contents of salinity 35 and 45 groups increased on Days 10 and 20, and were significantly higher than that of the control group (P<0.05), but ATP contents of all treatment groups were decreased on Day 30. The activities of T-SOD and CAT in treatment groups were higher than the control groups, and the activities showed an upward trend with the increase of experimental time. The results show that high-salinity stress was not conducive to the growth and survival of L. vannamei, but it could activate the antioxidant activity by increasing the activity of antioxidant enzymes, and significantly affect the lipid metabolism of L. vannamei.