| Morphological and toxicity characteristics of Pseudo-nitzschia pungens strain PP0201-01 isolated from the East China Sea |
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| 作者姓名： | 李爱峰 于仁成 王云峰 颜天 周名江 |
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| 作者单位： | [1]Key Laboratory of Marine Ecology＇ and Environment Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Graduate School, Chinese Academy of Sciences, Beijing 100039, China |
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| 基金项目： | Supported by the projects of Ministry of Science and Technology (No.2001CB409704 and No. 2001BA804A20), and the innovation project ofthe Institute of Oceanology, CAS (No. 200223107) |
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| 摘 要： | The morphology and toxicity of Pseudo-nitzschia strain PP0201-01, isolated near the estuary of Changjiang （Yangtze） River in Year 2002 and tentatively identified as Pseudo-nitzschia pungens, were studied. The strain was identified morphologically as P. pungens using scanning electronic microscope （SEM）. The analytical results of LC-PDA （liquid chromatography with photodiode array detector）, LC-MS （liquid chromatography with mass detector）, and the toxicity data from mouse bioassay, showed that no domoic acid was produced by the strain. However, the mechanism of domoic acid production by Pseudo-nitzschia species is complicated. More works are needed to assess the toxic species and to evaluate the potential risk of amnesic shellfish poisoning （ASP） in China.
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| 关 键 词： | 形态特征 毒性 中国 东海 生物测定 硅藻 ASP 甲壳类动物 |
| 收稿时间： | 2005-03-11 |
| 修稿时间： | 2005-07-18 |
Morphological and toxicity characteristics of Pseudo-nitzschia pungens strain PP0201-01 isolated from the East China Sea |
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| LI Aifeng, YU Rencheng, WANG Yunfeng, YAN Tian, ZHOU Mingjiang.Morphological and toxicity characteristics of Pseudo-nitzschia pungens strain PP0201-01 isolated from the East China Sea[J].Chinese Journal of Oceanology and Limnology,2005,23(4):418-426. |
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| Authors: | LI Aifeng YU Rencheng WANG Yunfeng YAN Tian ZHOU Mingjiang |
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| Abstract: | The morphology and toxicity of Pseudo-nitzschia strain PP0201-01, isolated near the estuary of Changjiang (Yangtze) River in Year 2002 and tentatively identified as Pseudo-nitzschia pungens, were studied. The strain was identified morphologically as P. pungens using scanning electronic microscope (SEM). The ana- lytical results of LC-PDA (liquid chromatography with photodiode array detector), LC-MS (liquid chromatogra- phy with mass detector), and the toxicity data from mouse bioassay, showed that no domoic acid was produced by the strain. However, the mechanism of domoic acid production by Pseudo-nitzschia species is complicated. More works are needed to assess the toxic species and to evaluate the potential risk of amnesic shellfish poison- ing (ASP) in China. |
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| Keywords: | domoic acid Pseudo-nitzschia pungens scanning electronic microscope (SEM) mouse bioassay LC-UV LC-MS |
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