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Adapting the CHEMTAX method for assessing phytoplankton taxonomic composition in Southeastern U.S. estuaries |
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| Authors: | Alan J Lewitus David L White Raphael G Tymowski Mark E Geesey Sabrina N Hymel Peter A Noble |
| Institution: | 1. Bell W. Baruch Institute for Marine and Coastal Sciences, University of South Carolina, and Marine Resources Research Institute, South Carolina Department of Natural Resources, Hollings Marine Laboratory, 331 Ft. Johnson Road, 29412, South Carolina, Charleston 2. Marine Science Program, University of South Carolina, 29208, Columbia, South Carolina 4. Baruch Marine Laboratory, Belle W. Baruch Institute for Marine and Coastal Sciences, University of South Carolina, 29442, Georgetown, South Carolina 5. Grice Marine Laboratory, College of Charleston, 295 Ft. Johnson Rd., 29412, Charleston, South Carolina 7. Civil and Environmental Engineering Department, University of Washington, Seattle, Washington, 98195 |
| Abstract: | CHEMTAX is a matrix factorization program used to derive taxonomic structure of phytoplankton from photosynthetic pigment vitios. The program was originally developed from and applied to the analysis of oceanic phytoplankton assemblages. We found that application of the original CHEMTAX reference matrix to southeastern United States estuarine systems produced inaccurate results, as verified by microscopy. Modification of the matrix, based primarily on the pigment ratios of 33 estuarine isolates, improved the predictive capabilities of CHEMTAX for our samples. Limitations of the method included an overstimation of diatom biomass (due to the inability to differentiate diatoms from taxa with chloroplasts derived from diatom endosymbionts, notably some dinoflagellates) and a tendency to exclude some raphidophyte species. In complement with microscopic verification, the method was shown to improve assessment of phytoplankton taxonomic composition. |
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