Effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics during assays of oil spill bioremediation: A microcosm study |
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| Institution: | 1. Department of Civil Engineering and Architecture, - University of Catania, Via A. Doria, 6, - 95125 Catania, Italy;2. Laboratori Nazionali del Sud, – Istituto Nazionale di Fisica Nucleare (INFN), Via S. Sofia, 62, - 95125 Catania, Italy;3. Faculty of Engineering and Architecture, Kore University of Enna, Cittadella Universitaria, 94100 Enna, Italy;4. Department of Chemical Sciences, - University of Catania, Via A. Doria, 6, - 95125 Catania, Italy;5. Agenzia Regionale per la Protezione Ambientale (ARPA), Struttura territoriale di Siracusa, Via Bufardeci, 22, - 96100 Siracusa, Italy |
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| Abstract: | Microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics. An experimental seawater microcosm, supplemented with crude oil and EPS2003 (SW + OIL + EPS2003), was monitored for 15 days and compared to control microcosm (only oil-polluted seawater, SW + OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out during all experimentation period. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil degradation was monitored by GC–MS analysis. Bioemulsificant addition stimulated an increase of the total bacterial abundance, change in the community structure and activity. The bioemulsificant also increased of 5 times the oil biodegradation rate.The data obtained from microcosm experiment indicated that EPS2003 could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process. |
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