Pyrene exposure influences the craniofacial cartilage development of Sebastiscus marmoratus embryos |
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| Affiliation: | 1. Key Laboratory of Ministry of Education for Subtropical Wetland Ecosystem Research, School of Life Sciences, Xiamen University, Xiamen, China;2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China;1. Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-8506, Japan;2. Facul. Of Science, Toho University, Funabashi, 274-8510, Japan;1. Mizuki biotech, Co., Ltd., 1-1 Hyakunenkouen, Kurume, Fukuoka 839-0864, Japan;2. Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan;3. Department of Biological and Chemical Systems Engineering, National Institute of Technology, Kumamoto College, 2627 Hirayama-shinmachi, Yatsushiro, Kumamoto 866-8501, Japan;4. Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan;5. Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Higashi-ku, Tsukide, Kumamoto 862-8502, Japan;1. Southern California Coastal Water Research Project, Costa Mesa, CA, USA;2. University of California, Berkeley, Berkeley, CA, USA;3. University of Massachusetts Boston, Boston, MA, USA;4. Mississippi State University, Starkville, MS, USA;5. US Army Engineer Research and Development Center, Vicksburg, MS, USA;6. University of Florida, Gainesville, FL, USA;7. Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Mérida, Mexico;8. Agilent Technologies, Santa Clara, CA, USA;9. Strand Scientific Intelligence Inc., San Francisco, CA, USA;10. Environment Canada, North Vancouver, B.C., Canada;11. Environment Canada, Moncton, NB, Canada;1. Shell Health, Shell International bv, P.O. Box 162, 2501 AN The Hague, The Netherlands;2. Division of Toxicology, Wageningen University, Wageningen, The Netherlands;1. Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil;2. Department of Chemistry, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil |
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| Abstract: | Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants, which are known carcinogens and teratogens. However, the toxicity of PAHs during skeletal development and the mechanism involved are not completely clear. In the present study, rockfish (Sebastiscus marmoratus) embryos were exposed to pyrene (Pyr) for 7 days at 0.5, 5 and 50 nM which resulted in craniofacial skeleton deformities. Pyr exposure for 6 days reduced the expression of PCNA, Col2a1 and Sox9 in the craniofacial skeleton revealed using in situ hybridization. These results suggest that Pyr exposure impairs skeleton development via disrupting the proliferation of the chondrocytes. At the same time, Pyr exposure reduced the expression of lox1 and inhibited the activity of lysyl oxidase, which is the key enzyme controlling the collagen cross-linking, and which might therefore have been one of the reasons for the deformative Meckel's cartilage (lower jaw). |
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