Protein expression from zooplankton communities in a metal contaminated NW mediterranean coastal ecosystem |
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| Institution: | 1. Laboratoire Processus de Transfert et d’Echanges dans l’Environnement (EA 3819), Université du Sud Toulon – Var, BP 20132, 83 957 La Garde Cedex, France;2. Plate-forme d’Analyse Protéomique Paris Sud-Ouest, INRA, Unité Mixte de Recherche en Génétique Végétale (UMR 320), Ferme du Moulon, 91 190 Gif-sur-Yvette, France;1. Institute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung, 202-24, Taiwan;1. Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, 202-24, Taiwan;1. Institute for Marine and Antarctic Studies, University of Tasmania, Private Bag 49, Hobart, Tasmania 7001, Australia;2. CSIRO Oceans and Atmosphere Flagship, Castray Esplanade, Hobart, Tasmania 7001, Australia;3. School of Aquatic and Fishery Sciences, University of Washington, Box 305520, Seattle, WA 98195, USA;4. School of Economics and Finance, University of Tasmania, Private Bag 85, Hobart, Tasmania 7001, Australia;1. Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, 27570 Bremerhaven, Germany;2. University of Cologne, Institute for Geology and Mineralogy, 50674 Cologne, Germany;1. Department of Pediatrics, University of California, Los Angeles, Los Angeles, California;2. Department of Bioengineering, University of California, Los Angeles, Los Angeles, California;3. Department of Medicine, University of California, Los Angeles, Los Angeles, California;4. Department of Physiology, University of California, Los Angeles, Los Angeles, California;5. Department of Orthopaedic Surgery, University of California, Los Angeles, Los Angeles, California;1. Section of Animal Biology, Department of Biology, Faculty of Sciences, University of Patras, GR 26 500, Greece;2. Laboratory of Biochemical Engineering and Environmental Technology, Department of Chemical Engineering, University of Patras, 1 Karatheodori St., University Campus, GR 26 500, Patras, Greece;3. Institute of Chemical Engineering Sciences, Foundation of Research & Technology Hellas (ICEHT/FORTH), 10 Stadiou St., Platani, GR 26 504, Patras, Greece;1. Institute of Biology, Pedagogical University of Cracow, Podbrzezie 3, 31-054 Krakow, Poland;2. Division of Parasitology, Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Wroc?aw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland |
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| Abstract: | Bidimensional and monodimensional polyacrylamide gel electrophoresis were used to study protein expression from zooplankton collected in thirteen stations of Toulon Bay (NW Mediterranean). In this ecosystem, Little Bay showed higher trace metal concentrations (13.5–23.8 nM for Cu, 0.73–1.24 nM for Pb, 27.8–58.7 nM for Zn) than Large Bay (Cu 2.2–15.6 nM; Pb 0.19–0.78 nM; Zn 9.0–38.8 nM). Trace metals positively correlated (p < 0.05) with expression of four zooplankton proteins (MW in kDa/pI: 25.0/5.6; 48.8/4.1; 38.2/4.4; 38.3/5.8) and with biomass of Oithona nana, predominant copepod in Little Bay. Sequencing by LC–MS/MS putatively provided zooplankton identity of these proteins: they were cytoskeleton actin, except one protein that was the chaperone calreticulin. We suggest that actin and calreticulin could be regarded as zooplankton markers of metal stress and be involved in a possible tolerance of O. nana to contamination, contributing to its development in a marine perturbed ecosystem. |
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